This study presents cryo-electron microscopy structures of the type I-E Cascade and provides insights into PAM-dependent R-loop formation, Cas3 recruitment and substrate handover. In type I CRISPR–Cas ...

The varied landscape of the region, from the jagged peaks of the North Cascades to the pebbled beaches of Fidalgo Island, is often cited as a reason Skagit County residents love living here. The ...